Thermo Fisher Scientific HEC1 Monoclonal Antibody (9G3.23)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 6 publications 6 publications

Flow Cytometry (Flow)

Assay-dependent

-

Immunoprecipitation (IP)

1-5 µg/mL

-

in situ PLA (PLA)

Assay-dependent

-

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Non-human primate, Pig, Rodent

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

9G3.23

Immunogen

Partial human HEC1 protein consisting of amino acids 56-642. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HEC1,uniProtId:O14777-1,ncbiNodeId:9606,antigenRange:56-642,antigenLength:642,antigenImageFileName:MA1-23308_HEC1_O14777-1_House_mouse.svg,antigenImageFileNamePDP:MA1-23308_HEC1_O14777-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2149871

Product Specific Information

Positive control of HELA WCE suggested.

Target Information

Hec1 is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.