Thermo Fisher Scientific HEC1 Monoclonal Antibody (9G3.23)
HEC1 단백질을 인식하는 마우스 단클론 항체로 Western blot, IHC, ICC/IF 등 다양한 응용에 사용 가능. Protein A로 정제된 액상 형태이며, 1 mg/mL 농도로 제공. 인간, 설치류 등 다양한 종에 반응하며 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 2 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 6 publications |
| Flow Cytometry (Flow) | Assay-dependent | – |
| Immunoprecipitation (IP) | 1–5 µg/mL | – |
| in situ PLA (PLA) | Assay-dependent | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Hamster, Human, Mouse, Non-human primate, Pig, Rodent |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9G3.23 |
| Immunogen | Partial human HEC1 protein consisting of amino acids 56–642 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2149871 |
Product Specific Information
Positive control of HeLa WCE suggested.
Target Information
Hec1 is one of several proteins involved in spindle checkpoint signaling.
This surveillance mechanism ensures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until proper bipolar attachment of chromosomes is achieved.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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