Thermo Fisher Scientific RAD52 Monoclonal Antibody (5H9)
RAD52 단백질을 인식하는 Thermo Fisher Scientific의 Mouse monoclonal 항체(클론 5H9). Western blot 및 Immunocytochemistry에 적합하며, HeLa 세포 용해물을 양성 대조군으로 권장. 단백질 G로 정제된 액상 항체로, 1 mg/mL 농도 및 PBS/20% glycerol buffer에 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5H9 |
| Immunogen | Human Rad52 recombinant protein expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 20% glycerol |
| Contains | No preservative |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2177449 |
Product Specific Information
- Recommended positive controls: HeLa whole cell lysate.
Target Information
DNA double-strand breaks can be generated by exposure to ionizing radiation or through endogenous cellular reactions. These breaks are generally repaired via homologous recombination (HR) or non-homologous end joining (NHEJ).
Homologous recombination requires the activity of the RAD52 epistasis group, including RAD50, RAD51, RAD52, RAD54, RDH54/TID1, RAD55, RAD57, RAD59, MRE11, and XRS2.
RAD52 can catalyze strand annealing reactions and form oligomers that attach to ends of single-stranded DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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