Thermo Fisher Scientific DDB1 Monoclonal Antibody (1322)
Human DDB1 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot 및 ELISA에 사용 가능. 고순도 Protein G 정제, 3 mg/mL 농도. PBS(pH 7.2) 버퍼, 보존제 없음. 연구용으로 T47D 및 MCF-7 세포 추출물에 적합.
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Thermo Fisher Scientific DDB1 Monoclonal Antibody (Clone 1322)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1322 |
| Immunogen | Synthetic peptide conjugated to protein carrier. The mice were immunized with a combination of N & C peptide (Swiss Prot ID: Q16531) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 3 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.2 |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_558609 |
Product Specific Information
- Recommended positive controls: T47D and MCF-7 whole cell lysate
- Store product as a concentrated solution
- Centrifuge briefly prior to opening the vial
Target Information
The DNA damage-binding protein from HeLa cells is associated with polypeptides of relative mass 124,000 (DDB1) and 41,000 (DDB2) as determined by SDS-polyacrylamide gels. To test whether the DNA-repair defect in the subset of XPE patients that lack DNA damage-binding activity is caused by a defect in DDB, Keeney et al. (1994) injected purified human DDB protein into XPE cells.
Usage Notes
For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.
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