Thermo Fisher Scientific SARS-CoV-2 Spike Protein S1/S2 (aa11-1208), His Tag Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Virus

Expression System

HEK293 cells

Amino acid sequence

SARS-CoV-2 Spike Protein S1+S2, amino acids Val11-Gln1208 (Accession # YP_009724390.1) (s-ECD region) with a C-terminal His-tag

Tag

His-tag

Class

Recombinant

Type

Protein

Purity

>97% by SDS-PAGE

Endotoxin concentration

<0.1 EU/µg

Activity

ED50 = 0.15-9.92 ng/mL; determined by the binding of immobilized SARS-CoV-2 Spike Protein S1+S2 recombinant protein (2 µg/mL) to human ACE2 recombinant protein in a functional ELISA.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-80°C

Shipping conditions

Dry ice

Product Specific Information

Storage: This product is stable at &plusmin; -70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

Target Information

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. The CoV Spike (S) protein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of antibodies, entry inhibitors and vaccines. Of note is the Spike protein receptor-binding domain (RBD, S-RBD) in SARS-CoV-2 Spike protein which binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibits significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD in comparison. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. SARS-CoV-2 S is 1273 amino acids long and consists of a signal peptide (amino acids 1-13) located at the N-terminus, the S1 subunit (14-685 residues), and the S2 subunit (686-1273 residues); the last two regions are responsible for receptor binding and membrane fusion, respectively. The S-ECD is the extracellular domain of the Spike protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.