Thermo Fisher Scientific SARS-CoV-2 Spike Protein S1/S2 (aa11-1208), His Tag Recombinant Protein
SARS-CoV-2 Spike Protein S1/S2 재조합 단백질로, HEK293 세포에서 발현된 His-tag 형태입니다. ELISA 및 Western blot 등 다양한 분석에 활용 가능하며, >97% 순도와 낮은 엔도톡신 수준을 제공합니다. ACE2 결합 활성이 확인된 고품질 연구용 시약입니다.
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Applications
Western Blot (WB)
- Assay-dependent
ELISA
- Assay-dependent
Control (Ctrl)
- Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Virus |
| Expression System | HEK293 cells |
| Amino Acid Sequence | SARS-CoV-2 Spike Protein S1+S2, amino acids Val11–Gln1208 (Accession # YP_009724390.1), s-ECD region with C-terminal His-tag |
| Tag | His-tag |
| Class | Recombinant |
| Type | Protein |
| Purity | >97% by SDS-PAGE |
| Endotoxin Concentration | <0.1 EU/µg |
| Activity | ED50 = 0.15–9.92 ng/mL; determined by the binding of immobilized SARS-CoV-2 Spike Protein S1+S2 recombinant protein (2 µg/mL) to human ACE2 recombinant protein in a functional ELISA |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -80°C |
| Shipping Conditions | Dry ice |
Product Specific Information
Storage: This product is stable at ± -70°C for up to 6 months from the date of receipt.
For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.
Avoid repeated freeze-thaw cycles.
Target Information
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2), also known as 2019-nCoV, causes illnesses ranging from mild cold-like symptoms to severe respiratory distress and death.
The Spike (S) protein is crucial for viral attachment, fusion, and entry, and serves as a target for antibody, inhibitor, and vaccine development.
The receptor-binding domain (RBD) of the Spike protein binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors, exhibiting higher affinity than the SARS-CoV RBD.
S1 contains the RBD that specifically binds ACE2 on target cells.
SARS-CoV-2 S protein consists of:
- Signal peptide (aa 1–13)
- S1 subunit (aa 14–685)
- S2 subunit (aa 686–1273)
The S-ECD is the extracellular domain of the Spike protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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