Thermo Fisher Scientific SARS-CoV-2 Spike Protein (RBD) (aa319-541), His Tag Recombinant Protein
SARS-CoV-2 Spike Protein RBD 영역(aa319-541)으로 구성된 재조합 단백질. HEK293 세포에서 발현되며 His-tag 부착. >95% 순도, ELISA 및 SPR 등 기능 분석에 사용 가능. ACE2 결합 활성 확인됨. 연구용으로만 사용.
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Applications
- Western Blot (WB): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
- Surface Plasmon Resonance (SPR): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Virus |
| Expression System | HEK293 cells |
| Amino Acid Sequence | SARS-CoV-2 Spike Protein RBD, amino acids Arg319–Phe541 (Accession # YP_009724390.1) (s-RBD region) with a C-terminal His-tag |
| Tag | His-tag |
| Molecular Weight | 25.9 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95% by SDS-PAGE; >95% by HPLC |
| Endotoxin Concentration | <1.0 EU/µg |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Spike RBD-His at 2 µg/mL (100 µL/well) can bind Recombinant Human ACE2 with a linear range of 0.15–4.53 ng/mL. Immobilized human ACE2 on COOH Chip can bind SARS-CoV-2 Spike RBD Protein with an affinity constant of 36.7 nM as determined in a SPR assay (Nicoya OpenSPR). |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
Product Specific Information
- Reconstitution: Reconstitute to a concentration of 0.1–0.5 mg/mL in sterile distilled water. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2–8°C for up to 1 week.
- Description: Recombinant 2019-nCoV Spike RBD-His Protein is produced by HEK293 cells expression system.
Target Information
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2), also known as 2019-nCoV, is a virus that causes illnesses ranging from mild cold-like symptoms to severe respiratory distress and death. The CoV Spike (S) protein plays a key role in viral attachment, fusion, and entry, serving as a target for antibody, inhibitor, and vaccine development.
The Spike protein receptor-binding domain (RBD, S-RBD) binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibits significantly higher binding affinity to ACE2 compared to SARS-CoV RBD. The S1 subunit contains the RBD that specifically interacts with ACE2 receptors on target cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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