Thermo Fisher Scientific SARS-CoV-2 Spike Protein (RBD) (aa319-541), His Tag Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Surface plasmon resonance (SPR)

Assay-dependent

-

Product Specifications

Species

Virus

Expression System

HEK293 cells

Amino acid sequence

SARS-CoV-2 Spike Protein RBD, amino acids Arg319-Phe541 (Accession # YP_009724390.1) (s-RBD region) with a C-terminal His-tag

Tag

His-tag

Molecular weight

25.9 kDa

Class

Recombinant

Type

Protein

Purity

>95% by SDS-PAGE; >95% by HPLC

Endotoxin concentration

<1.0 EU/µg

Activity

Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Spike RBD-His at 2 µg/mL (100 µL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-4.53 ng/mL.
Immobilized human ACE2 on COOH Chip, can bind SARS-COV-2 Spike RBD Protein with an affinity constant of 36.7 nM as determined in a SPR assay (Nicoya OpenSPR).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

Product Specific Information

Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Description: Recombinant 2019-nCoV Spike RBD-His Protein is produced by HEK293 cells expression system.

Target Information

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. The CoV Spike (S) protein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of antibodies, entry inhibitors and vaccines. Of note is the Spike protein receptor-binding domain (RBD, S-RBD) in SARS-CoV-2 Spike protein which binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibits significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD in comparison. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.