Thermo Fisher Scientific SARS-CoV-2 Spike Protein (RBD) (aa319-541), mFc Tag Recombinant Protein
SARS-CoV-2 스파이크 단백질 RBD(aa319-541) 영역을 포함한 재조합 단백질로, mFc 태그를 보유. ELISA, Western blot, SPR 등 다양한 분석에 사용 가능. HEK293 세포에서 발현되었으며, >95% 순도와 낮은 엔도톡신 수준을 보장. 연구용으로만 사용.
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Applications
- Western Blot (WB): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
- Surface Plasmon Resonance (SPR): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Virus |
| Expression System | HEK293 cells |
| Amino Acid Sequence | SARS-CoV-2 Spike Protein RBD, amino acids Arg319–Phe541 with a C-terminal mFc-tag |
| Tag | Fc-tag |
| Molecular Weight | 50.8 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95% by SDS-PAGE |
| Endotoxin Concentration | <0.1 EU/µg |
| Activity | Measured by binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 Protein at 2 µg/mL (100 µL/well) binds Recombinant SARS-CoV-2 Spike RBD-mFc Protein. ED50 = 2.58 ng/mL. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
Product Specific Information
- Animal Origin Free
- Storage and Reconstitution:
Store lyophilized protein at -20°C to -80°C for long-term storage.
Reconstitute to 0.1–0.5 mg/mL in sterile distilled water.
After reconstitution, stable for 3 months at -20°C or up to 1 week at 2–8°C.
Target Information
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2), also known as 2019-nCoV, causes illnesses ranging from mild cold-like symptoms to severe respiratory distress.
The CoV Spike (S) protein is critical for viral attachment, fusion, and entry, serving as a target for antibody, inhibitor, and vaccine development.
The receptor-binding domain (RBD, S-RBD) binds strongly to human and bat ACE2 receptors, showing higher affinity than SARS-CoV RBD.
S1 contains the RBD that specifically binds to ACE2 receptors on target cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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