Thermo Fisher Scientific Phospho-LAT (Tyr255) Polyclonal Antibody
Phospho-LAT (Tyr255) 폴리클로날 항체로, 인간·마우스·랫 시료에서 LAT 단백질의 Tyr255 인산화를 검출. Western blot에 적합하며, 항원 친화 크로마토그래피로 정제됨. 고순도(>95%)와 안정적인 보관 조건 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human LAT (Isoform Long) around the phosphorylation site of Tyrosine 255 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2554021 |
Product Specific Information
- Detects endogenous LAT protein at approximately 38 kDa.
- Purity >95% by SDS-PAGE.
Target Information
LAT (Linker for Activation of T cells) is a 36–38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells, and platelets.
After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules.
LAT is essential for TCR-dependent T cell and FcεRI-dependent mast cell activation, as well as for maturation of early thymocytes. It also plays a role in NK cell signaling and platelet activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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