Thermo Fisher Scientific Phospho-SHP2 (Tyr542) Polyclonal Antibody
Phospho-SHP2 (Tyr542) 폴리클로날 항체로, 인산화된 SHP2 단백질의 Tyr542 잔기를 특이적으로 인식합니다. Western blot, IHC, ICC/IF에 사용 가능하며, 사람, 생쥐, 랫트 시료에 반응합니다. PBS와 50% 글리세롤에 보관되며 -20°C에서 안정합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human PTPN11 (Accession Q06124), corresponding to amino acid residues around phosphorylated Tyr546. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2899277 |
Product Specific Information
This antibody detects endogenous levels of SHP2 only when phosphorylated at Tyr546, which site has historically been referenced as Tyr542.
Target Information
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate various cellular processes such as cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains that mediate phospho-tyrosine binding and substrate interaction. It is widely expressed and regulates multiple signaling events critical for mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are associated with Noonan syndrome and acute myeloid leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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