Thermo Fisher Scientific Phospho-MAPKAP1 (Thr86) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

Antibody detects endogenous levels of Sin1 only when phosphorylated at Thr86.

Target Information

Sin1 was initially identified as the human homolog of S. pombe SIN1. Recent evidence has shown that it is identical to Mip1, a protein that interacts with MEKK2, a member of the mitogen-activated protein kinase (MAPK) intracellular signaling network.
MAPKAP1 is thought to prevent MEKK2 activation and dimerization by forming a complex with inactive and non-phosphorylated MEKK2, thereby blocking the JNK1/2, ERK1/2, p38, and ERK5 MAPKs.
MAPKAP1 also plays a role in the TOR signaling process, which controls cell growth and proliferation in response to environmental cues such as nutrients, growth factors, and hormones.
Experiments have shown that MAPKAP1 helps maintain the TOR/rictor assembly but not the TOR/RAPTOR complex, enabling specific phosphorylation of Akt, a kinase that couples the growth factor-PI3K signaling pathway to the nutrient-regulated TOR signaling pathway.
Multiple alternatively spliced isoforms of MAPKAP1 have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.