Thermo Fisher Scientific Mouse anti-Human Kappa Light Chain Secondary Antibody, Biotin
인간 카파 라이트 체인 검출용 마우스 단클론 2차 항체로 Biotin 결합형입니다. WB, IHC, Flow, ELISA 등 다양한 응용에 적합하며, 높은 특이성과 감도를 제공합니다. Protein G 정제 및 PBS/BSA 완충액으로 안정적 보관이 가능합니다.
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Thermo Fisher Scientific Mouse anti-Human Kappa Light Chain Secondary Antibody, Biotin
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:400–1:1,600 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:400–1:800 |
| Flow Cytometry (Flow) | 1 µg/mL |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 2 µg |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Secondary Antibody |
| Clone | L1C1 |
| Immunogen | B lymphoma cells |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10984749 |
| Target | Kappa light chain |
| Antibody Form | Whole Antibody |
Additional Formats
- Alexa Fluor 488
- APC
- FITC
- PE
- Request custom conjugation available
Product Specific Information
By Western blot, MA5-12114 detects Human Ig kappa light chain at approximately 25 kDa in human samples. At high concentrations, the antibody may also detect heavy chain.
By ELISA and WB, MA5-12114 does not react with human Ig lambda light chain.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins. They are useful in detection, sorting, or purification of specific targets. Secondary antibodies increase versatility by enabling multiple detection systems (e.g., HRP, AP, fluorescence) and enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
These antibodies are typically generated by immunizing host animals with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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