Thermo Fisher Scientific Mouse anti-Human Kappa Light Chain Secondary Antibody, Biotin

Applications

Tested Dilution

Western Blot (WB)

1:400-1:1,600

Immunohistochemistry (Paraffin) (IHC (P))

1:400-1:800

Flow Cytometry (Flow)

1 µg/mL

ELISA (ELISA)

1 µg/mL

Immunoprecipitation (IP)

2 µg

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Secondary Antibody

Clone

L1C1

Immunogen

B lymphoma cells

Conjugate

Biotin Biotin Biotin

View additional formats

• [Alexa Fluor 488 ](/antibody/secondary/product-name/Human Kappa Light Chain/conjugates/Alexa Fluor 488/host/Mouse/)
• [APC ](/antibody/secondary/product-name/Human Kappa Light Chain/conjugates/APC/host/Mouse/)
• [FITC](/antibody/secondary/product-name/Human Kappa Light Chain/conjugates/FITC/host/Mouse/)
• [PE ](/antibody/secondary/product-name/Human Kappa Light Chain/conjugates/PE/host/Mouse/)
• Request custom conjugation

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10984749

Target

Kappa light chain

Antibody Form

Whole Antibody

Product Specific Information

By Western blot, MA5-12114 detects Human Ig kappa light chain chain at ~25kD in human samples. At high concentrations, the antibody will also detect heavy chain.

By ELISA and WB, MA5-12114 does not react with human Ig lambda light chain.

Target Information

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.