Thermo Fisher Scientific POLB Monoclonal Antibody (61)

Applications

Tested Dilution

Publications

Western Blot (WB)

2-4 µg/mL

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

-

View 2 publications 2 publications

Product Specifications

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

61

Immunogen

DNA polymerase beta protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10975730

Product Specific Information

MA5-12086 targets DNA Polymerase beta in WB applications and shows reactivity with Human samples.

The MA5-12086 immunogen is dNA polymerase beta protein.

Target Information

DNA polymerase beta comprises an amino-terminal 8- kDa domain and a carboxy-terminal 31- kDa domain. The N-terminal ssDNA binding domain has a deoxyribose phosphodiesterase activity while the C-terminal domain has a nucleotidyltransferase activity. Mammalian DNA polymerase beta, a DNA repair polymerase, is constitutively expressed in cultured cells, but treatment of cells with the DNA-alkylating agents such as N-methyl-N`-nitro-N-nitrosoguanidine (MNNG) or methyl methanesulfonate up-regulates beta-pol level. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.