Thermo Fisher Scientific c-Myc Monoclonal Antibody (9E10.3)
Thermo Fisher Scientific의 c-Myc Monoclonal Antibody (Clone 9E10.3)는 인간 c-Myc 단백질을 인식하며 WB, IHC, ICC/IF, IP 등 다양한 응용에 적합합니다. 단일클론 항체로 높은 특이성과 재현성을 제공하며, 연구용으로 세포 증식 및 종양 관련 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:50–1:200 | View 15 publications |
| Immunohistochemistry (IHC) | – | View 7 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:25–1:50 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100 | View 6 publications |
| Flow Cytometry (Flow) | Assay-dependent | View 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | View 3 publications |
| Immunomicroscopy (IM) | Assay-dependent | – |
| Miscellaneous PubMed (Misc) | – | View 18 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Tag, Xenopus, Yeast |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9E10.3 |
| Immunogen | Synthetic peptide, aa 408–439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from C-terminus of human c-Myc |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11009013 |
Additional Formats
Product Specific Information
- MA5-12080 detects c-Myc in human samples.
- Does not react with mouse, rat, or chicken (others not tested).
- Suitable for FACS, IF, IHC (P), IM, IP, and WB applications.
- Immunogen: synthetic peptide, aa 408–439, from the C-terminus of human c-Myc.
Target Information
The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is commonly activated in various tumor cells and plays a crucial role in cellular proliferation, differentiation, apoptosis, and cell cycle progression.
Phosphorylation at Thr58/Ser62 by kinases such as GSK3, CDK, ERK2, and JNK regulates its activity.
c-Myc is essential for vasculogenesis and angiogenesis in tumor development.
Aberrant expression or amplification of c-Myc is associated with numerous human cancers, including lung, breast, colon, and Burkitt’s lymphoma.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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