Thermo Fisher Scientific Cytokeratin 18 Monoclonal Antibody (DC10), Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 8 publications 8 publications

Immunohistochemistry (IHC)

-

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

-

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

DC10

Immunogen

Human breast cancer PMC 42 cells if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cytokeratin 18,uniProtId:P05783-1,ncbiNodeId:9606,antigenRange:1-430,antigenLength:430,antigenImageFileName:MA5-12101_Cytokeratin_18_P05783-1_House_mouse.svg,antigenImageFileNamePDP:MA5-12101_Cytokeratin_18_P05783-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10986090

Product Specific Information

MA5-12101 targets Cytokeratin 18 in IF, IHC (P), and WB applications and shows reactivity with Human samples.

The MA5-12101 immunogen is human breast cancer PMC 42 cells.

Target Information

Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Further, cytokeratin 18 is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas. Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for the cytokeratin 18 gene. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.