Thermo Fisher Scientific EEA1 Monoclonal Antibody (F.43.1)
EEA1 단백질을 인식하는 F.43.1 클론의 Rabbit Monoclonal Antibody로, WB, ICC/IF, IP 등 다양한 응용에 적합. 인간, 마우스, 랫트에 반응하며 고순도 Affinity Chromatography로 정제됨. 44 µg/mL 농도의 액상 형태로 -20°C에서 보관.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 3 publications |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | View 8 publications |
| Immunoprecipitation (IP) | 1:100 | View publications |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Dog, Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | F.43.1 |
| Immunogen | Synthetic peptide corresponding to residues surrounding Ser70 of human EEA1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 44 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10985824 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Early endosomal antigen 1 (EEA1) is a 162 kDa membrane-bound protein specific to the early endosome and essential for fusion between early endocytic vesicles. Early endosomes fuse with endocytic vesicles to redistribute extracellular compounds. EEA1 contains zinc-finger-like domains in both amino and carboxyl-terminal regions, similar to those found in nucleic acid-binding proteins. The carboxyl-terminal zinc-finger-like domain is conserved in other non-nuclear proteins involved in intracellular trafficking and is a true zinc-binding domain critical for EEA1 localization. Membrane association of EEA1 depends on phosphatidyl 3-kinase activity; inhibitors cause EEA1 to dissociate from early endosomes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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