Thermo Fisher Scientific EEA1 Monoclonal Antibody (F.43.1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:50-1:200

View 8 publications 8 publications

Immunoprecipitation (IP)

1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Dog, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

F.43.1

Immunogen

Synthetic peptide corresponding to residues surrounding Ser70 of human EEA1 protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EEA1,uniProtId:Q15075-1,ncbiNodeId:9606,antigenRange:70,antigenLength:1411,antigenImageFileName:MA5-14794_EEA1_Q15075-1_Rabbit.svg,antigenImageFileNamePDP:MA5-14794_EEA1_Q15075-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

44 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10985824

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc-finger-like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl-terminal domains of EEA1. The carboxyl-terminal zinc-finger-like-domain is conserved in several other non-nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc-binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3-kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.