Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), eFluor 450, eBioscience

Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), eFluor 450, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin, Brilliant UV/Violet/Yellow series
• FITC, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436–780 series
• NovaFluor Blue, Red, Violet, Yellow series
• Functional Grade
• Request custom conjugation

Product Specific Information

The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein expressed by B cells during all developmental stages except plasma cells. Follicular dendritic cells also express CD19. Together with CD21, CD81, Leu13, and MHC class II, CD19 forms a multimolecular complex associated with the BCR, mediating signaling that induces tyrosine phosphorylation, calcium flux, and B cell proliferation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested by flow cytometric analysis of human mononuclear cells at 5 µL (0.5 µg) per test.
A test is defined as the amount of antibody that stains a cell sample in a final volume of 100 µL. Cell number may range from 10⁵ to 10⁸ cells/test.

Fluorochrome Information:
eFluor 450 is an alternative to Pacific Blue, excited at 405 nm and emits at 445 nm. Ensure instrument compatibility with Violet laser detection.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on 100% of peripheral B cells and may also appear on certain myeloid leukemia cells. CD19 acts as a critical regulator of B cell activation and proliferation, forming complexes with the B cell receptor to lower activation thresholds. Mutations lead to hypogammaglobulinemia, while overexpression results in B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지