Thermo Fisher Scientific Ezrin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:50-1:100

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 350-470 of human Ezrinin (NP_0033702) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Ezrin,uniProtId:P15311-1,ncbiNodeId:9606,antigenRange:350-470,antigenLength:586,antigenImageFileName:PA5-87489_Ezrin_P15311-1_Rabbit.svg,antigenImageFileNamePDP:PA5-87489_Ezrin_P15311-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.41 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804194

Product Specific Information

Immunogen sequence: RLQDYEEKTK KAERELSEQI QRALQLEEER KRAQEEAERL EADRMAALRA KEELERQAVD QIKSQEQLAA ELAEYTAKIA LLEEARRRKE DEVEEWQHRA KEAQDDLVKT KEELHLVMTA P; Positive Samples: M21, SW480, U-251MG, 293T, Mouse intestine, Mouse brain, Rat brain; Cellular Location: Apical cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, cell cortex, cytoskeleton, microvillus membrane, ruffle membrane

Target Information

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.