Thermo Fisher Scientific Ezrin Polyclonal Antibody
Ezrin 단백질을 인식하는 Rabbit Polyclonal 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot, ICC/IF, ELISA, IP에 적합하며, 액상 형태로 제공됩니다. 세포막 및 세포골격 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein corresponding to amino acids 350–470 of human Ezrin (NP_0033702) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.41 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804194 |
Product Specific Information
Immunogen sequence:
RLQDYEEKTK KAERELSEQI QRALQLEEER KRAQEEAERL EADRMAALRA KEELERQAVD QIKSQEQLAA ELAEYTAKIA LLEEARRRKE DEVEEWQHRA KEAQDDLVKT KEELHLVMTA P
Positive Samples:
M21, SW480, U-251MG, 293T, Mouse intestine, Mouse brain, Rat brain
Cellular Location:
Apical cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, Cell cortex, Cytoskeleton, Microvillus membrane, Ruffle membrane
Target Information
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, it serves as an intermediate between the plasma membrane and the actin cytoskeleton. It plays a key role in cell surface structure adhesion, migration, and organization, and has been implicated in various human cancers. A pseudogene on chromosome 3 and alternatively spliced variants have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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