Thermo Fisher Scientific uNOS Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 8 publications 8 publications

Immunohistochemistry (IHC)

-

View 16 publications 16 publications

Immunohistochemistry (Frozen) (IHC (F))

1:100

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:200

View 10 publications 10 publications

Product Specifications

Species Reactivity

Arthropod, Bovine, Fruit fly, Human, Mouse, Pig, Rat

Published species

Arthropod, Cnidarian, Crab, Fish, Fruit fly, Human, Insect, Mollusc, Mouse, Pig, Rabbit, Rat, Shark

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues D Q(1113) K R Y H E D I F G(1122) of murine iNOS and bNOS. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:uNOS,uniProtId:Q9Z0J4-1,ncbiNodeId:10090,antigenRange:1113-1122,antigenLength:1429,antigenImageFileName:PA1-039_uNOS_Q9Z0J4-1_Rabbit.svg,antigenImageFileNamePDP:PA1-039_uNOS_Q9Z0J4-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325776

Product Specific Information

PA1-039 detects mouse macrophage inducible nitric oxide synthase (iNOS); rat, bovine, Drosophila, and porcine brain nitric oxide synthase (bNOS); human, porcine and bovine epithelial nitric oxide synthase (eNOS).

PA1-039 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~135 kDa protein representing mouse macrophage iNOS; an ~155 kDa protein representing rat, bovine and porcine bNOS; ~140 kDa protein representing porcine and bovine eNOS. Immunohistochemical staining of NOS in Drosophila optic tissue with PA1-039 results in staining of the optic nerve. In immunohistochemistry procedures PA1-039 also stains NOS in human endothelial cells, macrophages, and peripheral neural tract cells.

The PA1-039 immunogen is a synthetic peptide corresponding to residues D Q(1113) K R Y H E D I F G(1122) of mouse iNOS and bNOS. This sequence is highly conserved among the different NOS isoforms.

Target Information

NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); induciblr NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). This antibody reacts with iNOS, bNOS and eNOS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.