Thermo Fisher Scientific NPM1 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

PA1-029 detects nucleophosmin (NPM) from human, rat, and mouse samples.
It has been successfully used in Western blot and immunofluorescence procedures.
By Western blot, this antibody detects an approximately 38 kDa protein corresponding to human, mouse, and rat nucleophosmin in various cell lysates.
The immunogen is a synthetic peptide corresponding to amino acid residues 23–38 and 226–240 from human NPM.

Target Information

NPM1 (Nucleophosmin 1, B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in ribosome assembly and transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and p53 regulation.
It continuously shuttles between the nucleus, cytoplasm, and nucleolus, chaperoning core histones from the nucleus to the cytoplasm.
NPM1 binds nucleic acids, prevents protein aggregation, protects enzymes during thermal denaturation, and assists renaturation of chemically denatured proteins.
It is associated with the centrosome and acts as a substrate for CDK2/cyclin E during centrosome duplication.
NPM1 is also implicated in tumor-associated chromosome translocations forming fusion proteins such as NPM-ALK, NPM-RAR, and NPM-MLF1.
Additionally, NPM1 sequesters the tumor suppressor RF in the nucleolus, protecting it from degradation.
Dysfunction of NPM1 is associated with diseases such as acute myeloid leukemia and lymphomatoid papulosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.