Thermo Fisher Scientific PSD93 Polyclonal Antibody

Applications

Product Specifications

Product Specific Information

PA1-043 detects Post Synaptic Density 93 (PSD-93) from rat and human tissues. This antibody does not detect other synapse-associated protein family members.

PA1-043 has been successfully used in Western blot and immunohistochemistry procedures.
By Western blot, this antibody detects an ~110 kDa protein representing PSD-93 in rat brain extract.
Immunohistochemical staining of PSD-93 in rat cerebellum with PA1-043 yields a pattern consistent with post-synaptic localization.

The PA1-043 immunogen is a synthetic peptide corresponding to residues N(352) K L C D K P A S P R H Y S P(366) of human PSD-93. This sequence is completely conserved in human PSD-93.
PA1-043 immunizing peptide (Cat. # PEP-021) is available for use in neutralization and control experiments.

Target Information

PSD-93, also known as chapsyn-110, is one of a family of plasma membrane-associated proteins found in synaptic junctions.
It is uniquely expressed in Purkinje neuron cell bodies and dendrites. PSD-93 has three ~90 amino acid repeats called PDZ domains, a single interior SH3 domain, and a carboxyl-terminal guanylate kinase homology (GuK) domain that is enzymatically inactive.

PDZ-domain interactions are thought to play a role in receptor and channel clustering, contributing to neuronal plasticity.
PSD-93 participates in clustering of certain proteins, including NMDA receptors and shaker-type potassium channels at the synaptic membrane.
NMDA receptors and shaker-type potassium channels share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif, enabling PDZ-domain binding.
Neuronal nitric oxide synthase (nNOS), which lacks this motif but has its own PDZ domain, can interact with PSD-93 through a pseudo-homotypic PDZ-PDZ interaction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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