Thermo Fisher Scientific Human Fc gamma RIIB (CD32b) His-tag Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

HEK293 cells

Amino acid sequence

Human CD32b, amino acids Ala46-Pro217 (Accession # NP_001002274.1) with an N-terminal signal peptide and a C-terminal His-tag

Tag

His-tag

Molecular weight

20.8 kDa

Class

Recombinant

Type

Protein

Purity

≥95 % as determined by SDS-PAGE. ≥95 % as determined by SEC-HPLC.

Endotoxin concentration

<1.0 EU/µg

Activity

Loaded Recombinant Human CD32B/Fcgr2 Protein, His Tag on His1K Biosensor, can bind Rituximab with an affinity constant of 1.13 µM as determined in BLI assay.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80

Contains

no preservative

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient

Product Specific Information

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Target Information

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.