Thermo Fisher Scientific CD40 Monoclonal Antibody (3/23)

Applications and Tested Dilution

Product Specifications

Additional Formats:

• PE Conjugate (MA1-81163)
• Request custom conjugation

Product Specific Information

• Low-endotoxin, preservative-free formulation (endotoxin level <0.01 EU/µg).
• For FACS analysis: use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
• Rat anti-Mouse CD40 antibody, clone 3/23, recognizes the murine CD40 cell surface glycoprotein.

Target Information

CD40 is a single-chain glycoprotein belonging to the TNF/NGF superfamily, showing homology to CD30. It is expressed on all B cells except plasma cells, and also on certain epithelial cells, carcinomas, and dendritic cells. CD40 plays an essential role in immune and inflammatory responses, including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.

The AT-hook transcription factor AKNA regulates CD40 expression, influencing cell interactions. TNFR2 interacts with CD40 to mediate signal transduction. The CD40–CD40L interaction is necessary for amyloid-beta-induced microglial activation, implicated in early Alzheimer’s disease pathogenesis. Two alternatively spliced transcript variants encoding distinct isoforms have been reported. CD40 dysfunction is associated with Type 3 Hyper-IgM immunodeficiency and CD40 ligand deficiency.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.