Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), Brilliant Violet 480, eBioscience

Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), Brilliant Violet 480, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 615, 661, 737
• Brilliant Violet 605, 650, 711
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available

Product Specific Information

The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein expressed by B cells throughout development except plasma cells. Follicular dendritic cells also express CD19. CD19 forms a complex with CD21, CD81, Leu13, and MHC class II, associating with BCR to mediate signaling through tyrosine phosphorylation, calcium flux, and proliferation of B cells.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells at 5 µL (0.25 µg) per test.
Brilliant Violet™ 480 emits at 479 nm and is suitable for cytometers with a violet (405 nm) laser.
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.
Not compatible with methanol-based fixation.
Excitation: 440 nm; Emission: 479 nm; Laser: Violet Laser.
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains, expressed on all peripheral B cells. It is also found on certain myeloid leukemia cells. CD19 regulates B cell proliferation and is expressed in all B cell precursor leukemias. It functions as a signal-amplifying coreceptor for the B cell receptor (BCR) and is a central regulatory component in B cell signaling. Mutations cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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