Thermo Fisher Scientific PMCA1 ATPase Polyclonal Antibody

Thermo Fisher Scientific PMCA1 ATPase Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

PA1-914 detects calcium pump of the plasma membrane 1a (PMCA1a ATPase) and 1b from rat, human, and porcine tissues.
It has been successfully used in Western blot, immunohistochemistry (paraffin and frozen), and ELISA procedures.
By Western blot, this antibody detects ~134 kDa and ~130 kDa proteins representing PMCA1a and 1b ATPase from rat, human, and porcine samples.
The immunogen is a synthetic peptide corresponding to residues A(5) N N S V A Y S G V K N S I K E A N(22) of rat PMCA1 ATPase.

Target Information

The calcium pump of the plasma membrane (PMCA ATPase) transports calcium from the cytosol to the extracellular space.
This membrane-bound enzyme is related to other ATPases such as SERCA and the sodium/potassium pump.
Four genes encode PMCA ATPase, and 20 isoforms are generated by alternative splicing.
mRNA studies show gene products 1 and 4 are expressed in most tissues, while 2 and 3 are tissue-specific.
In the pancreas, beta cells express only the 4b isoform, while alpha and gamma cells express both 4a and 4b.
Different splice variants exhibit varying affinities for calcium and calmodulin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.