Thermo Fisher Scientific PMCA1 ATPase Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

-

Immunohistochemistry (Frozen) (IHC (F))

1:20

-

Immunocytochemistry (ICC/IF)

-

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Pig, Rat, Human

Published species

Human, Mouse, Non-human primate, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to the residues A(5) N N S V A Y S G V K N S I K E A N(22) of rat PMCA1 ATPase. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PMCA1 ATPase,uniProtId:P11505-3,ncbiNodeId:10114,antigenRange:5-22,antigenLength:1220,antigenImageFileName:PA1-914_PMCA1_ATPase_P11505-3_Rabbit.svg,antigenImageFileNamePDP:PA1-914_PMCA1_ATPase_P11505-3_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2061563

Product Specific Information

PA1-914 detects calcium pump of the plasma membrane 1a (PMCA1a ATPase) and 1b from rat, human and porcine tissues.

PA1-914 has been successfully used in Western blot, immunohistochemistry (paraffin and frozen), and ELISA procedures. By Western blot, this antibody detects ~134 kDa and ~130 kDa proteins representing PMCA1a and 1b ATPase from rat, human and porcine samples.

The PA1-914 immunogen is a synthetic peptide corresponding to the residues A(5) N N S V A Y S G V K N S I K E A N(22) of rat PMCA1 ATPase.

Target Information

The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/potassium pump. There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.