Thermo Fisher Scientific Bromodeoxyuridine Monoclonal Antibody (BU6-4)

Applications

Tested Dilution

Publications

ELISA (ELISA)

5 µg/mL

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

BU6-4

Immunogen

Bromodeoxyuridine-BSA conjugate.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5-1.0 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute in 50 µL of distilled water (2 mg/mL final concentration). If further dilution is required, dilute just prior to use.

Target Information

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.