Thermo Fisher Scientific H3K4me2 Monoclonal Antibody (T.767.5)
H3K4me2 특이적 단클론 항체로 Western blot, IHC, ICC, IP 등 다양한 응용에 적합. Rabbit IgG 기반, Affinity chromatography로 정제. 인간, 마우스, 설치류, 영장류 시료에 반응. -20°C 보관, 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:750–1:3000 |
| Immunocytochemistry (ICC/IF) | 1:250 |
| Immunoprecipitation (IP) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | T.767.5 |
| Immunogen | Synthetic peptide corresponding to the amino terminus of histone H3 in which Lys4 is di-methylated |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 51 µg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.01M HEPES (pH 7.5), 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_10984942 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with di-methylated or tri-methylated histone H3 Lys9, Lys27, Lys36, or histone H4 Lys20.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
Post-translationally, histones are modified in various ways to alter chromatin structure or facilitate the binding of specific transcription factors. The N-terminal tail of histone H3 can undergo several post-translational modifications, including methylation or acetylation of lysine and arginine residues, and phosphorylation of serine or threonine, influencing multiple cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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