Thermo Fisher Scientific Phospho-BTK/ITK (Tyr551, Tyr511) Monoclonal Antibody (M4G3LN), eBioscience
BTK 및 ITK의 인산화 형태(Tyr551, Tyr511)를 인식하는 마우스 단클론 항체로, 인간 및 마우스 시료에 반응합니다. Western blot, IHC, Flow cytometry에 적합하며, 고순도(>90%)로 제조되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.1 µg/mL | - |
| Flow Cytometry (Flow) | Assay-Dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M4G3LN |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865315 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Conjugated Formats
Product Specific Information
This M4G3LN monoclonal antibody recognizes human and mouse Bruton’s tyrosine kinase (BTK) and interleukin-2-inducible tyrosine kinase (ITK) when phosphorylated on Y551 and Y511, respectively.
Tec family kinases, including BTK and ITK, are critical for activation of phospholipase C and calcium-dependent signaling following antigen receptor triggering. BTK is involved in B cell signaling via TLR4 co-receptor RP105/CD180. Deficiency or mutation in BTK or ITK leads to defects in B or T cell development and signaling, such as X-linked agammaglobulinemia.
Specificity of the M4G3LN clone was confirmed by ELISA, flow cytometry, and western blotting.
Applications Reported:
- Intracellular staining (flow cytometry)
- Western blotting
- Immunohistochemistry (formalin-fixed paraffin-embedded tissue)
Applications Tested:
- Immunoblotting of stimulated Daudi B cells: ≤5 µg/mL
- IHC (formalin-fixed paraffin-embedded human tissue, high pH antigen retrieval): ≤0.1 µg/mL
It is recommended to titrate the antibody for optimal performance.
Target Information
Tec family kinases (including BTK and ITK) are receptor-proximal kinases expressed in B and T cells.
BTK mediates B cell activation, while ITK functions in T cell signaling.
Mutations or deletions in BTK result in X-linked agammaglobulinemia, and ITK deficiencies impair T cell signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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