Thermo Fisher Scientific CD266 (TWEAK Receptor) Monoclonal Antibody (ITEM-4), eBioscience
CD266(TWEAK Receptor) 인식 ITEM-4 단클론 항체로 인간 및 마우스 Fn14 단백질 검출에 적합. Flow cytometry, WB, IHC 등 다양한 응용 가능. 0.5 mg/mL 농도의 액상 제품으로 PBS buffer 및 sodium azide 함유. 연구용으로만 사용.
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Thermo Fisher Scientific CD266 (TWEAK Receptor) Monoclonal Antibody (ITEM-4), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | View 3 publications |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | View publications |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | View 5 publications |
| Neutralization (Neu) | - | View 2 publications |
| Functional Assay (Functional) | - | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ITEM-4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_468541 |
Additional Formats Available:
Product Specific Information
The ITEM-4 antibody reacts with human TWEAK Receptor/Fn14 (fibroblast growth factor-inducible 14 kDa protein). Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but lacks a death domain (DD) cytoplasmic region. Fn14 plays roles in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis.
TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis, which can be blocked by an anti-TWEAK antibody (CARL-1). Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs. Fn14 mRNA expression has been identified during liver regeneration. ITEM-4 cross-reacts with mouse Fn14, which is constitutively expressed by the Colon 26 cell line.
The ITEM-4 antibody exhibits blocking functionality.
Applications Reported: Flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections.
Applications Tested: Flow cytometric analysis on human and mouse Fn14 transfected cells. Recommended ≤1 µg/test in 100 µL volume (10⁵–10⁸ cells/test). Optimize titration per assay.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
TWEAK is a member of the TNF family, initially described as a weak inducer of apoptosis. Its receptor, TweakR, was cloned from a HUVEC cDNA library and belongs to the TNF receptor superfamily. TweakR can initiate proliferative signals via TRAF1, TRAF2, and TRAF3 binding. TweakR mRNA is upregulated during tissue regeneration in the aorta and liver and may contribute to vascular cell migration and angiogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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