Thermo Fisher Scientific CD266 (TWEAK Receptor) Monoclonal Antibody (ITEM-4), eBioscience

Thermo Fisher Scientific CD266 (TWEAK Receptor) Monoclonal Antibody (ITEM-4), eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available:

• Biotin
• Functional Grade
• PE
• Request custom conjugation

Product Specific Information

The ITEM-4 antibody reacts with human TWEAK Receptor/Fn14 (fibroblast growth factor-inducible 14 kDa protein). Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but lacks a death domain (DD) cytoplasmic region. Fn14 plays roles in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis.

TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis, which can be blocked by an anti-TWEAK antibody (CARL-1). Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs. Fn14 mRNA expression has been identified during liver regeneration. ITEM-4 cross-reacts with mouse Fn14, which is constitutively expressed by the Colon 26 cell line.

The ITEM-4 antibody exhibits blocking functionality.

Applications Reported: Flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections.
Applications Tested: Flow cytometric analysis on human and mouse Fn14 transfected cells. Recommended ≤1 µg/test in 100 µL volume (10⁵–10⁸ cells/test). Optimize titration per assay.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

TWEAK is a member of the TNF family, initially described as a weak inducer of apoptosis. Its receptor, TweakR, was cloned from a HUVEC cDNA library and belongs to the TNF receptor superfamily. TweakR can initiate proliferative signals via TRAF1, TRAF2, and TRAF3 binding. TweakR mRNA is upregulated during tissue regeneration in the aorta and liver and may contribute to vascular cell migration and angiogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.