Thermo Fisher Scientific Cytokeratin 7 Monoclonal Antibody (LP5K), eBioscience
Human cytokeratin 7 인식하는 LP5K 단클론 항체로, WB 및 ICC/IF에 적합. 0.5 mg/mL 액상 형태이며 PBS 버퍼와 sodium azide 포함. 높은 순도(>90%)로 제조되어 세포 구조 연구 및 암 진단 마커 연구에 활용 가능.
- 판매단위
- pk
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Thermo Fisher Scientific Cytokeratin 7 Monoclonal Antibody (LP5K), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View 1 publication |
| Flow Cytometry (Flow) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | LP5K |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10698018 |
Additional Formats
Product Specific Information
This LP5K monoclonal antibody reacts with human cytokeratin 7 (K7), a 54-kDa type II (basic) keratin expressed in simple epithelia, mesothelium, urothelium, and pseudostratified epithelium. Expression in gastric foveolar, intestinal, and stratified squamous epithelia is very low or undetectable.
Cytokeratins form the intracellular cytoskeletal network maintaining cell and tissue integrity. Most carcinomas express cytokeratin 7, and its co-expression with cytokeratin 20 serves as a diagnostic marker for various carcinomas.
Applications Reported: Suitable for immunoblotting (WB) and immunocytochemistry (ICC).
Applications Tested: Tested by immunofluorescent staining of paraformaldehyde-fixed and permeabilized cells. Recommended concentration ≤10 µg/mL; titration advised for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Cytokeratin 7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. It is involved in translational regulation of human papillomavirus type 16 E7 mRNA (HPV16 E7).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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