Thermo Fisher Scientific Fumarase Monoclonal Antibody (3D5A3G7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

ELISA (ELISA)

1:5,000-1:10,000

-

Immunoprecipitation (IP)

0.2-1 µL/mg of lysate

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3D5A3G7

Immunogen

Recombinant Human Fumarate Hydratase/FH protein (Ala45-Lys510) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Fumarase,uniProtId:P07954-1,ncbiNodeId:9606,antigenRange:45-510,antigenLength:510,antigenImageFileName:MA5-29275_Fumarase_P07954-1_House_mouse.svg,antigenImageFileNamePDP:MA5-29275_Fumarase_P07954-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2785169

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human Fumarate Hydratase/FH.

Target Information

The protein encoded by this gene is an enzymatic component of the tricarboxylic acid cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.