Thermo Fisher Scientific NFkB p65 Recombinant Superclonal Antibody (4-2HCLC)
NFkB p65를 검출하기 위한 Thermo Fisher Scientific의 Recombinant Superclonal Rabbit Antibody. 고감도와 높은 특이성을 제공하며, WB, IHC, ICC, ELISA, ChIP 등 다양한 응용에 적합. 재조합 기술로 로트 간 일관성 확보, 인간 및 생쥐 반응성.
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Thermo Fisher Scientific NFkB p65 Recombinant Superclonal Antibody (4-2HCLC)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–1 µg/mL | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:100 | - |
| Immunocytochemistry (ICC/IF) | 1 µg/mL | View 2 publications |
| ELISA | Assay-dependent | - |
| ChIP assay (ChIP) | 3 µg | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Superclonal |
| Type | Antibody |
| Clone | 4-2HCLC |
| Immunogen | Recombinant protein corresponding to amino acids 375–551 of human NFkB p65 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532539 |
Product Specific Information
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They comprise multiple different recombinant monoclonal antibodies, combining polyclonal sensitivity with monoclonal specificity and recombinant consistency.
These antibodies recognize multiple epitopes on the target, providing higher detection sensitivity for low-abundance proteins. Each lot contains a defined mixture of light and heavy chains, eliminating variability typical of polyclonal production.
Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and performance remain unchanged.
Target Information
Nuclear factor kB p65 (NF-kB p65), encoded by the RELA gene on chromosome 11 in humans, is also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A).
It belongs to the Rel family of proteins and forms part of the NF-kB complex, heterodimerizing with p50 or p52 subunits.
Upon TNF alpha binding to its receptor, IKK is phosphorylated, leading to phosphorylation and degradation of I kB. NF-kB p65 plays a crucial role in transcription of the immunoglobulin k (kappa) gene in mature B-lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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