Thermo Fisher Scientific Phospho-LCK (Ser59) Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

This antibody detects endogenous levels of LCK only when phosphorylated at Ser59.

Target Information

LCK is a member of the Src-family tyrosine kinases essential for signaling through the T-cell receptor (TCR) complex. Upon TCR activation, LCK phosphorylates ITAM motifs in the zeta subunits, creating binding sites for the SH2 domains of ZAP70, which is subsequently phosphorylated and activated by LCK. Activated ZAP70 phosphorylates adaptor LAT, initiating downstream signaling.
LCK is mainly localized at the plasma membrane but also associates with the Golgi apparatus, potentially contributing to Raf activation under weak TCR stimulation. It contains N-terminal myristylation and palmitylation sites, a PTK domain, and SH2/SH3 domains for protein-protein interactions.
LCK is implicated in apoptosis regulation (excluding death receptor pathways) and diseases such as Immunodeficiency 22 and CD45 deficiency. Alternative splicing produces multiple isoforms of the LCK protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.