Thermo Fisher Scientific c-Fos Monoclonal Antibody (5B11), PerCP

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000

Product Specifications

Product Specific Information

A 1:1,000 dilution of MA5-45891 was sufficient for detection of c-Fos in 10 µg of serum-starved HeLa cells treated with PMA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects a 62 kDa band. The observed molecular weight is higher than the predicted 41 kDa band due to post-translational modifications.

Target Information

FBJ murine osteosarcoma viral oncogene homolog, commonly known as Cellular proto-oncogene Fos (c-Fos), belongs to the fos transcription factor family and is encoded by the C-FOS gene on chromosome 14.
c-Fos heterodimerizes with c-Jun to form the Activator Protein-1 (AP-1) transcription factor, which regulates genes involved in cellular signal transduction, proliferation, and differentiation.
c-Fos is phosphorylated by various stimuli including growth factors and insulin at multiple sites. The best-known upstream kinase for phosphorylation at Threonine 232 is ERK MAPK, which regulates c-Fos nuclear localization and transcriptional activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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