Thermo Fisher Scientific c-Fos Monoclonal Antibody (12G11), APC
APC 형광 표지된 c-Fos 단클론 항체로 인간 c-Fos 단백질 검출에 적합. Western blot 및 IHC에 사용 가능. 높은 특이성과 신뢰성으로 세포 신호전달 연구에 활용. 연구용으로만 사용.
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Thermo Fisher Scientific c-Fos Monoclonal Antibody (12G11), APC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 12G11 |
| Immunogen | Synthetic peptide from the N-terminal of Human c-Fos (aa. 280–380) |
| Conjugate | APC (Allophycocyanin) |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
A 1:1,000 dilution of MA5-45892 was sufficient for detection of c-Fos in 10 µg of serum-starved HeLa cells treated with PMA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects 62 kDa band. Observed molecular weight of band is higher than predicted 41 kDa band due to post-translational modifications.
Target Information
FBJ murine osteosarcoma viral oncogene homolog, commonly known as Cellular protooncogene Fos (c-Fos), belongs to the Fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14.
c-Fos heterodimerizes with c-Jun to form the Activator Protein-1 (AP-1) transcription factor, which regulates transcription of several genes involved in cellular signal transduction, proliferation, and differentiation.
c-Fos is phosphorylated by various stimuli including growth factors and insulin at multiple sites. The upstream kinase ERK MAPK phosphorylates threonine 232, regulating nuclear localization and transcriptional activity of c-Fos.
Usage Restriction
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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