Thermo Fisher Scientific c-Fos Monoclonal Antibody (5B11), FITC
FITC로 표지된 c-Fos 단클론 항체(클론 5B11)로, Western blot 등에서 인간 c-Fos 단백질 검출에 적합. 1:1,000 희석으로 높은 특이성과 감도를 제공. 액상 형태, 4°C 암소 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5B11 |
| Immunogen | Synthetic peptide from the N-terminal of Human c-Fos (aa. 280–380) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
A 1:1,000 dilution of MA5-45890 was sufficient for detection of c-Fos in 10 µg of serum-starved HeLa cells treated with PMA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects a 62 kDa band. The observed molecular weight is higher than the predicted 41 kDa band due to post-translational modifications.
Target Information
FBJ murine osteosarcoma viral oncogene homolog, commonly known as Cellular proto-oncogene Fos (c-Fos), belongs to the Fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14.
c-Fos heterodimerizes with c-Jun to form the Activator Protein-1 (AP-1) transcription factor, which regulates genes involved in signal transduction, cell proliferation, and differentiation.
c-Fos is phosphorylated by various stimuli, including growth factors and insulin, at multiple sites. The best-known upstream kinase for phosphorylation at Threonine 232 is ERK MAPK, which regulates the nuclear localization and transcriptional activity of c-Fos.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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