Thermo Fisher Scientific c-Fos Monoclonal Antibody (12G11), FITC
FITC 형광 표지된 c-Fos 단일클론 항체(12G11)로, Western blot 및 면역조직화학에 적합. 인간 c-Fos N-말단 펩타이드(aa 280–380)를 면역원으로 사용. 498/517 nm의 형광 특성, 1 mg/mL 액상 형태로 제공. 4°C 암소 보관.
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Thermo Fisher Scientific c-Fos Monoclonal Antibody (12G11), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 12G11 |
| Immunogen | Synthetic peptide from the N-terminal of Human c-Fos (aa 280–380) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | 4°C, store in dark |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
A 1:1,000 dilution of MA5-45893 was sufficient for detection of c-Fos in 10 µg of serum-starved HeLa cells treated with PMA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects 62 kDa band. Observed molecular weight of band is higher than predicted 41 kDa due to post-translational modifications.
Target Information
FBJ murine osteosarcoma viral oncogene homolog (c-Fos) belongs to the Fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14.
c-Fos heterodimerizes with c-Jun to form the Activator Protein-1 (AP-1) transcription factor, regulating transcription of genes involved in signal transduction, proliferation, and differentiation.
It is phosphorylated by various stimuli including growth factors and insulin at multiple sites. The ERK MAPK kinase phosphorylates threonine 232, regulating nuclear localization and transcriptional activity of c-Fos.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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