Thermo Fisher Scientific c-Fos Monoclonal Antibody (12G11), FITC

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (IHC)

1:100

-

Product Specifications

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

12G11

Immunogen

Synthetic peptide from the N-terminal of Human c-Fos (aa. 280-380) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:c-Fos,uniProtId:P01100-1,ncbiNodeId:9606,antigenRange:280-380,antigenLength:380,antigenImageFileName:MA5-45893_c-Fos_P01100-1_House_mouse.svg,antigenImageFileNamePDP:MA5-45893_c-Fos_P01100-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

A 1:1,000 dilution of MA5-45893 was sufficient for detection of c-fos in 10 µg of serum starved HeLa cells treated with PMA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects 62 kDa band. Observed molecular weight of band is higher than predicted 41 kDa band due to post translational modifications.

Target Information

FBJ murine osteosarcoma viral oncogene homolog is commonly known as Cellular protooncogene Fos (c-Fos). It belongs to the fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14. c-Fos heterodimerizes with c-Jun to form the Activator Protein -1 (AP-1) transcription factor which regulates transcription of several genes that play an important role in cellular signal transduction, cell proliferation and differentiation. c-Fos has been shown to be phosphorylated by various stimuli including growth factors and insulin at atleast seven different sites. The best known upstream kinase for phosphorylation at Threonine 232 is ERK MAPK which regulates localization of c-Fos to the nucleus and is thus important for c-Fos induced transcriptional activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.