Thermo Fisher Scientific IL-1 alpha Polyclonal Antibody, PeproTech
Rabbit polyclonal antibody against Human IL-1 alpha for WB, ELISA, and immunostaining. Affinity-purified, lyophilized form with high specificity and sensitivity. Ideal for detecting IL-1α in inflammation and cytokine studies. For research use only.
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Thermo Fisher Scientific IL-1 alpha Polyclonal Antibody, PeproTech
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 3 publications |
| ELISA | 0.5–2.0 µg/mL | View publications |
| Neutralization (Neu) | – | View 4 publications |
| In vitro Assay (IV) | – | View 1 publication |
| Immunostaining (IS) | – | View 3 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Rat |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human IL-1alpha |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929867 |
Product Specific Information
AA Sequence of recombinant protein:
SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-1alpha. Anti-Human IL-1alpha-specific antibody was purified by affinity chromatography employing an immobilized Human IL-1alpha matrix.
Sandwich ELISA:
To detect Human IL-1alpha by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL is required. In conjunction with PeproTech Biotinylated Anti-Human IL-1alpha (500-P21ABt) as a detection antibody, this allows detection of at least 0.2–0.4 ng/well of Recombinant Human IL-1alpha.
Western Blot:
To detect Human IL-1alpha by Western Blot, use at 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for Recombinant Human IL-1alpha is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It is encoded by the IL1A gene and signals through IL-1RI and IL-1RII receptors, shared with IL-1 beta. Located on chromosome 2q13, IL-1 alpha plays a crucial role in innate immune defense by triggering production of other proinflammatory cytokines and initiating acute-phase responses.
IL-1 alpha activity is regulated by IL-1 Receptor Antagonist (IL-1RA), which competitively inhibits receptor binding. It is involved in immune responses, inflammation, and hematopoiesis, and is processed and released upon cell injury, inducing apoptosis. The IL1A gene forms part of a cytokine gene cluster and modulates NF-kappaB and MAPK activity, stimulating IL6 and COX-2 expression and nitric oxide production. Elevated IL-1 alpha levels are linked to chronic inflammatory diseases such as rheumatoid arthritis, psoriasis, and multiple sclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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