Thermo Fisher Scientific EpCAM Recombinant Rabbit Monoclonal Antibody (BLR077G)
EpCAM 단백질을 인식하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 등에 사용 가능. 인간 및 생쥐 반응성. HEK293 세포에서 발현된 정제 항체로, 비결합형 액상 형태이며 4°C에서 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 - 1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 - 1:500 |
| Flow Cytometry (Flow) | 0.5 µL per 1 x 10^6 cells |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR077G |
| Immunogen | Peptide representing a region between residue 264 and the C-terminus (residue 314) of human Epithelial Cell Adhesion Molecule (EpCAM). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 500 µg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 7.8–8.6, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in epithelial tissues with a molecular weight of approximately 40 kDa. It functions as a calcium-independent homotypic cell adhesion molecule and influences cell cycle, proliferation, and metabolism by inducing proto-oncogene c-myc and cell cycle regulators cyclin A and E. Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression of Ep-CAM is linked to enhanced epithelial proliferation and is a marker for epithelial-derived tumors. It is expressed on the baso-lateral cell surface in most simple epithelia and many carcinoma types, and can distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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