Thermo Fisher Scientific MYOD Monoclonal Antibody (5.8A)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 14 publications 14 publications

Immunohistochemistry (IHC)

-

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

-

Immunohistochemistry (Frozen) (IHC (F))

1:10-1:500

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

5 µg/mL

View 8 publications 8 publications

Flow Cytometry (Flow)

Assay-Dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

1 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

5.8A

Immunogen

Recombinant mouse MyoD.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2282434

Product Specific Information

The epitope of this antibody was mapped to a region within AA 180-189 of mouse MyoD.

Suggested positive control: Rh-30.

Target Information

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.