Thermo Fisher Scientific PARP1 (cleaved Asp214, Asp215) Monoclonal Antibody (194C1439)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

Immunocytochemistry (ICC/IF)

2-5 µg/mL

-

Flow Cytometry (Flow)

1-10 µg/million cells

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

194C1439

Immunogen

Synthetic peptide corresponding to residues near the 214/215-cleavage site of human PARP. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PARP1 (cleaved Asp214),uniProtId:P09874-1,ncbiNodeId:9606,antigenRange:215,antigenLength:1014,antigenImageFileName:MA1-41020_PARP1_cleaved_Asp214_P09874-1_House_mouse.svg,antigenImageFileNamePDP:MA1-41020_PARP1_cleaved_Asp214_P09874-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1077468

Product Specific Information

Suggested positive control: camptothecin treated HL-60 (12 hr).

Target Information

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.