Thermo Fisher Scientific PARP1 (cleaved Asp214, Asp215) Monoclonal Antibody (194C1439)
PARP1 단백질의 Asp214, Asp215 절단 부위를 인식하는 단클론 항체. Western blot, ICC/IF, Flow cytometry에 적합. Human 및 Mouse 시료 반응성. Protein G 정제, 1.0 mg/mL 농도의 액상 형태로 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 2–5 µg/mL |
| Flow Cytometry (Flow) | 1–10 µg/million cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 194C1439 |
| Immunogen | Synthetic peptide corresponding to residues near the 214/215 cleavage site of human PARP |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1077468 |
Product Specific Information
- Suggested positive control: camptothecin-treated HL-60 (12 hr)
Target Information
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyze the transfer of ADP-ribose to various nuclear protein acceptors.
Proteolysis of PARP to its stable 85 kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein.
Cleavage occurs between Asp216 and Gly217, a site conserved across species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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