Thermo Fisher Scientific GAPDH Recombinant Rabbit Monoclonal Antibody (RM114), Biotin
GAPDH 단백질을 인식하는 Biotin 결합 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, IP, ChIP 등 다양한 응용에 적합합니다. 높은 특이성과 일관된 로트 간 재현성을 제공하며, 단백질 로딩 컨트롤용으로 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Immunoprecipitation (IP) | 1:200 |
| ChIP assay (ChIP) | 1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | All, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM114 |
| Immunogen | A peptide corresponding to the C-terminus of GAPDH |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744964 |
Additional formats available:
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to rabbit immune repertoire
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme involved in glycolysis, existing as a tetramer of identical 37-kDa subunits. It catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphate in the presence of NADPH.
Beyond its glycolytic role, GAPDH is ubiquitously expressed and participates in:
- DNA replication and repair
- Nuclear RNA export
- Membrane fusion and microtubule bundling
- Apoptosis and neurodegenerative disease pathways
GAPDH interacts with amyloid precursor protein (linked to Alzheimer’s disease) and Huntingtin (linked to Huntington’s disease), as well as actin and tubulin. Due to its high expression in most tissues, GAPDH serves as a reliable protein loading control in Western blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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