Thermo Fisher Scientific GAPDH Recombinant Rabbit Monoclonal Antibody (RM114)
GAPDH 단백질을 검출하기 위한 Thermo Fisher Scientific의 재조합 토끼 단클론 항체입니다. Western blot, ICC/IF, IP, ChIP 등에 사용 가능하며 높은 특이성과 민감도를 제공합니다. HEK293 발현 시스템 기반으로 생산되어 로트 간 일관성이 우수합니다.
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Thermo Fisher Scientific GAPDH Recombinant Rabbit Monoclonal Antibody (RM114)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Immunoprecipitation (IP) | 1:200 |
| ChIP assay (ChIP) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | All, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM114 |
| Immunogen | Peptide corresponding to the C-terminus of GAPDH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744965 |
Additional Formats
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits and screen individual clones to select optimal candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme involved in glycolysis, existing as a tetramer of 37-kDa subunits. It catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphate in the presence of NADPH.
Beyond glycolysis, GAPDH participates in DNA replication, repair, RNA export, membrane fusion, and microtubule bundling. It is implicated in apoptosis, neurodegenerative diseases such as Alzheimer’s and Huntington’s, and interacts with proteins like amyloid precursor protein and Huntingtin.
Due to its ubiquitous expression, GAPDH serves as a reliable protein loading control in Western blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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