Thermo Fisher Scientific Phospho-CREB (Ser133) Recombinant Rabbit Monoclonal Antibody (B16H16L26)

Applications

• Western Blot (WB): 1–3 µg/mL

Product Specifications

Product Specific Information

Intact IgG appears on a non-reducing gel as a ~150 kDa band and upon reduction generates a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.

Advantages of recombinant rabbit monoclonal antibodies include:

• Superior specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity due to the larger rabbit immune repertoire

Target Information

CREB (Cyclic AMP response element binding protein) is a 43 kDa basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to various extracellular signals such as neurotransmitters, hormones, membrane depolarization, and growth factors.

Activation of CREB depends on phosphorylation at serine 133, mediated by kinases including p44/42 MAPK, p90RSK, p38 MAPK, and MSK1. Although CREB binds DNA regardless of phosphorylation, only the phosphorylated form functions as an active transcription factor.

CREB binding protein (CBP), a transcriptional coactivator, interacts with CREB near serine 133. CREB plays roles in circadian rhythm synchronization, adipocyte differentiation, and learning and memory. The human CREB gene is located on chromosome 2q. Dysregulation of CREB has been linked to diseases such as Alzheimer’s disease, histiocytoma, and soft tissue melanoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.