Thermo Fisher Scientific Phospho-c-Kit (Tyr703) Recombinant Rabbit Monoclonal Antibody (53H8L29)

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody is predicted to react with bovine, human, rat, and mouse based on sequence homology.

Intact IgG appears on a non-reducing gel as a ~150 kDa band and, upon reduction, generates a ~25 kDa light chain and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.

Advantages of recombinant rabbit monoclonal antibodies:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity due to larger rabbit immune repertoire

Target Information

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). It was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT and its ligand regulate growth and activation of various hematopoietic and non-hematopoietic cells.

Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Deregulation of KIT receptor TK, particularly the D816V activation loop mutation, is a therapeutic target for neoplastic mast cell growth.

c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells, and gastrointestinal tract Cajal cells. Upon binding of stem cell factor (SCF), c-Kit dimerizes, leading to receptor activation and autophosphorylation of tyrosine residues, including Tyr703, enabling Grb2 docking and activation of the Ras/ERK signaling pathway. SCF/c-Kit also activates PI3K, PLC-γ, and JAK/STAT pathways. Activation of c-Kit is essential for hematopoiesis, stem cell maintenance, and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.