Thermo Fisher Scientific LGR5 Monoclonal Antibody (UMAB210), UltraMAB
LGR5 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합. Human 및 Mouse 시료 반응성. 동결건조 형태로 제공되며, 재구성 시 1 mg/mL 농도로 사용 가능. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
| ChIP assay (ChIP) | Assay-Dependent |
| Peptide Array (Array) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UMAB210 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 22–543 of human LGR5 produced in HEK293 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
G protein-coupled receptor GPR49 (LGR5) is expressed in brain, skeletal muscle, placenta, and spinal cord.
It belongs to the GPCR family, characterized by seven transmembrane helices and existing in both active and inactive forms.
GPCRs mediate signaling through G-proteins and downstream effectors, playing crucial roles in physiological regulation.
Pharmacological research targeting GPCRs has led to the development of major drugs such as Zyprexa and Claritin.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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