Thermo Fisher Scientific WIBG Monoclonal Antibody (UMAB208), UltraMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

WIBG is a key regulator of the exon junction complex (EJC), a multiprotein complex that associates upstream of exon-exon junctions on mRNAs. It serves as a positional landmark for intron-exon structure and directs post-transcriptional processes such as mRNA export, nonsense-mediated mRNA decay (NMD), and translation.
Acts as an EJC disassembly factor, allowing translation-dependent EJC removal and recycling. Its association with the 40S ribosomal subunit prevents translation-independent EJC disassembly. WIBG interferes with NMD and enhances translation of spliced mRNAs by antagonizing EJC functions.
May bind RNA; however, RNA-binding relevance in vivo remains unclear. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.