Thermo Fisher Scientific Armenian Hamster IgG Isotype Control, PE

Applications

Tested Dilution

Flow Cytometry (Flow)

Assay-dependent

Control (Ctrl)

Assay-Dependent

Product Specifications

Host/Isotype

Armenian hamster / IgG

Class

Control

Type

Isotype Control

Immunogen

Trinitrophenol - KLH

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Lyophilized

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_2550637

Product Specific Information

PA5-33222 is suitable for use as a negative control for the measurement of non-specific binding of Armenian hamster monoclonal antibodies, of isotype IgG, in human, mouse or rat tissues.

Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4ºC in the dark.

In Flow cytometry, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Target Information

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.