Thermo Fisher Scientific Rat IgG2a Isotype Control

Applications

Tested Dilution

Flow Cytometry (Flow)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Control (Ctrl)

Assay-Dependent

Product Specifications

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Isotype Control

Immunogen

Human lymphocytes.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1% BSA, 5% sucrose

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2550628

Product Specific Information

PA5-33212 has been shown to be negative on all mouse cells and cell lines tested. This antibody recognizes a human cell surface marker, and therefore cannot be used as a negative control in this species Test results have shown that PA5-33212 is also suitable for use as a negative control with canine tissues.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Target Information

Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications. It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC). Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains. It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.