Thermo Fisher Scientific L1CAM Monoclonal Antibody (OTI2G9), TrueMAB
L1CAM 단백질을 인식하는 Mouse IgG1 단일클론 항체로 인간 및 개 시료에 반응합니다. Western blot, ICC/IF, Flow Cytometry에 사용 가능하며, 무보존제 PBS 버퍼에 동결건조 형태로 제공됩니다. 신경계 발달 관련 연구용으로 적합합니다.
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Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2G9 |
| Immunogen | Full length human recombinant protein of human L1CAM produced in HEK293T cell (Target: CD171 (L1CAM), UniProt ID: P32004-1, Antigen range: 1–1257) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
L1CAM/CD171 is an axonal glycoprotein of the immunoglobulin supergene family.
Its ectodomain contains multiple immunoglobulin-like and fibronectin type III domains, connected by a transmembrane sequence to a conserved cytoplasmic domain.
This molecule plays a crucial role in nervous system development, including neuronal migration and differentiation.
Mutations in the L1CAM gene cause three X-linked neurological syndromes collectively known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia, and hydrocephalus).
Alternative splicing of a neuron-specific exon is functionally significant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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