Thermo Fisher Scientific H3K14ac Recombinant Rabbit Monoclonal Antibody (JU43-26), ChIP-Verified
H3K14ac 표적을 인식하는 재조합 토끼 모노클로날 항체로, ChIP 검증 완료. Western blot, IHC, ICC, IP 등 다양한 응용 가능. 높은 특이성과 민감도, 로트 간 일관성, 동물 유래 성분 없음. 단기 4°C, 장기 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:500 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Immunoprecipitation (IP) | 1–2 µg/sample | - |
| ChIP assay (ChIP) | 1:100 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JU43-26 |
| Immunogen | Synthetic peptide within Human Histone H3 aa 1–50 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810090 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to rabbit immune repertoire
Click here for Master Lot linking information
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. Together with four core histone proteins, H3 binds to DNA forming the nucleosome structure. Histones play a central role in transcription regulation, DNA repair, replication, and chromosomal stability.
Post-translational modifications of histones, such as methylation, acetylation, and phosphorylation, influence chromatin structure and cellular processes. The N-terminal tail of histone H3 can undergo various modifications that affect transcription factor binding and chromatin dynamics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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