Thermo Fisher Scientific SDHB Recombinant Rabbit Monoclonal Antibody (JU02-33)
Western blot, IHC, Flow cytometry, IP 등 다양한 응용에 적합한 SDHB 단백질 검출용 항체. 재조합 토끼 단일클론으로 높은 특이성과 재현성을 제공. 인간, 마우스, 랫트 반응성. Protein A 정제 및 안정한 액상 형태로 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
| Immunoprecipitation (IP) | 1:10–1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JU02-33 |
| Immunogen | Synthetic peptide within Human SDHB (aa 201–250) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810092 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select optimal candidates for production.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
Complex II of the respiratory chain is involved in the oxidation of succinate and electron transfer from FADH to CoQ. It consists of four nuclear-encoded subunits located in the mitochondrial inner membrane.
The iron-sulfur subunit contains three cysteine-rich clusters forming the enzyme’s iron-sulfur centers.
Mutations in this gene are associated with paragangliomas and pheochromocytoma, linking mitochondrial dysfunction to tumorigenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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